Skip to main content
Fig. 1 | Journal of Experimental & Clinical Cancer Research

Fig. 1

From: CK2-mediated phosphorylation of Che-1/AATF is required for its pro-proliferative activity

Fig. 1

Che-1 depletion reduces cell proliferation. A Cell proliferation analysis (top) and WB analysis of total cell extracts (bottom) from the indicated cell lines transiently transfected with siRNA Control (siControl) or siRNA Che-1 (siChe-1) for 48 h. Bar plot shows the average of number of cells observed in these experiments (n = 3). B Two images representing nuclear morphology (left), corresponding fluorescence intensity analysis (middle) and WB analysis (right) of HCT116 cells transiently transfected with siControl or siChe-1. Nuclei were visualized by staining with Hoechst dye. Scale bar, 10 μm. Error bars represent the SD after combining the results of three different experiments. C Representative WB analysis with the indicated antibodies of nuclear extracts from HCT116 cells transfected as in B. D Representative immunofluorescence analysis (left) and corresponding fluorescence intensity analysis (right) of H3K9me3 expression in HCT116 cells transfected as in B. Nuclei were visualized by staining with Hoechst dye. Scale bar, 10 μm. E Representative WB analysis with the indicated antibodies of total cell extracts from the indicated cell lines transfected as in A (left). Relative H3 and H4 acetylated protein levels were calculated from three different experiments by densitometry (right). Statistical significance is indicated by asterisks as follow: *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001, n.s. = not significant

Back to article page