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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: CK2-mediated phosphorylation of Che-1/AATF is required for its pro-proliferative activity

Fig. 4

Che-1 and SV40 Large T antigen display similar functions. A Sequence alignment of Che-1 and SV40 Large T antigen (SV40 LT) proteins. Conserved serine residues are in red. B Schematic representation of SV40 LT point mutations (see Methods) at the indicated sites. C Cell proliferation analysis (left) and relative representative WB analysis (right) of HCT116 cells transfected with pSG5-SV40 LT wt, 3S mutant or empty vector (pcDNA3). Bar plot shows the average number of cells observed in these experiments (n = 3). D WB analysis with the indicated antibodies of total cell extracts from HCT116 cells transfected as in C. E Nuclear extracts from HCT116 cells transfected as in C and subjected to IP with SV40 antibody. Immunoprecipitated complexes were then analysed by WB with the indicated antibodies. Input corresponds to 10% of the nuclear extracts used for IP (left). Relative H3 binding was calculated from three different experiments by densitometry. H3 intensity was normalized to the one of SV40 wt or 3S mutant (right). Statistical significance is indicated by asterisks as follow: *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001, n.s. = not significant

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