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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: CK2-mediated phosphorylation of Che-1/AATF is required for its pro-proliferative activity

Fig. 5

CK2 phosphorylates Che-1. A Nuclear extracts from HCT116 cells subjected to IP with Che-1 antibody. Immunoprecipitated complexes were then analysed by WB with the indicated antibodies. Input corresponds to 10% of the nuclear extracts used for IP. B and C 2D-Gel electrophoresis of total cell extracts from HeLa cells treated or not with 80 μM TBB for 4 h (B) or transfected with siControl or siCK2 (C). D Representative immunofluorescence analysis of Che-1 expression in the indicated cell lines transiently transfected with siControl or siCK2. Scale bar, 10 μm. E Nuclear extracts from HCT116 cells transiently transfected as in D and subjected to IP with H3 antibody. Immunoprecipitated complexes were then analysed by WB with the indicated antibodies (left). Input corresponds to 10% of the nuclear extracts used for IP. Relative Che-1 binding was calculated from three different experiments by densitometry. Che-1 intensity was normalized to the one of H3 (right). Statistical significance is indicated by asterisks as follow: *P < 0.05, **P < 0.01, ***P < 0.005, ****P < 0.001, n.s. = not significant

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