Fig. 3

REG exerts potent anti-angiogenic effects in orthotopic CT26 tumors. a Tumor vascularization analyzed by DCE-MRI. Longitudinal effects on vascularization are shown by relative amplitude changes versus baseline (day 4). b Representative images. Left panel: T1-weighted MR images with overlayed amplitude parameter maps derived from DCE-MRI analyses (scale bar indicates arbitrary units); right panel: IF staining for CD31 (green), VEGFR2 (red) and nuclei (DAPI; blue). Yellow staining indicates overlapping signal for CD31 and VEGFR2. Scale bar: 100 µm. c Effects on tumor vasculature analyzed by immunofluorescence (IF) on frozen tumor sections for CD31 and VEGFR2 and quantified: mean values ± SD (n = 5–7) and individual values (dots). Statistical significance (^§day 7; ^#day 11; * day 14): *^/§/#p < 0.05; **^/§§/##p < 0.01; ***^/§§§/###p < 0.001; ****^{/§§§§/####}p < 0.0001