Fig. 5

Addition of aPD1 to REG leads to sustained inhibition of tumor growth and metastasis after discontinuation of therapy. a Study design. Gray arrowhead indicates imaging before first treatment for baseline determination. b Tumor growth curves, mean RTV given as a percentage of the baseline volumes ± SD (n = 7). Black arrow indicates treatment stop. Statistical significance: *p < 0.05; ***p < 0.001. c Representative images. Top panel: dissected tumors (arrow indicates tumor; * indicates cecum tissue); middle panel: T2-weighted MR images, white arrows indicate tumors; bottom panel: livers with metastases indicated by black arrows. Scale bar: 10 mm (MRI and ex vivo). d Number of mice with liver metastases vs total number of mice and percentages (number in %)