Fig. 2

Transcriptional and protein characterization of the activation state of the canonical Wnt pathway in sarcoma cell lines depending on EMX expression. A Characterization of the expression levels of EMX1, EMX2, CTNNB1 and TCF4 in primary sarcoma lines. B Characterization of the levels of EMX1, EMX2, and the WNT pathway factors β-CAT (β-catenin), p-β-CAT (p-β-catenin S33/S37/T41) and c-MYC in the primary sarcoma lines. C and D Effect of EMX1 and EMX2 overexpression on the WNT pathway determined by analyzing the negative regulators p-β-CAT, AXIN1 and GSK3-β and the positive regulators β-CAT, TCF4, c-MYC and WNT1 in the AA (C) and AW (D) model cell lines. E Effect of reduced EMX1/EMX2 expression on BG cells. SW480 cells (colorectal cancer line) were used as a positive cellular control for inactivation of the Wnt pathway. A Western blot experiment representative of 3 experiments performed independently in triplicate is shown