Skip to main content
Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Empty spiracles homeobox genes EMX1 and EMX2 regulate WNT pathway activation in sarcomagenesis

Fig. 3

Effects of EMX1 and EMX2 levels on the expression of target genes in the Wnt signaling pathway. A and B Relative mRNA levels of the AXIN1, GSK3β, WNT1, CTNNB1, TCF4 and CCND1 genes determined by qRT-qPCR in AA (A) and AW (B) cell lines. C Effects of reducing EMX1 and EMX2 expression on the expression of the same genes related to the Wnt signaling pathway in the BG cell line. Graphs of the expression levels (2-ΔCt) for total extracts of each cell line (ET), tumorspheres (TO) and xenotransplants (XEN). The mean of a minimum of 3 independent experiments performed in triplicate ± standard deviation are shown. Statistical analysis was performed with Student’s t test (* p < 0.05; ** p < 0.01; *** p < 0.001)

Back to article page