Fig. 4

Effects of overexpressing EMX1 and EMX2 in combination with constitutive activation of the Wnt pathway. A and C Relative mRNA levels (2-ΔCt) of the EMX1 (E1), EMX2 (E2) and CTNNB1 (β-CAT) genes determined by qRT-qPCR in models of overexpression of EMX1 or EMX2 and CTNNB1 in AA (A) and AW (C) cell lines. B and D Joint overexpression of EMX1, EMX2 and CTNNB1 in AA (B) and AW (D) cell lines. The mean of a minimum of 3 independent experiments performed in triplicate ± standard deviation are shown. Statistical analysis was performed with Student's t test (* p < 0.05; ** p < 0.01; *** p < 0.001). E and F Characterization of the protein levels of EMX1, EMX2 and the WNT pathway factors β-CAT (β-catenin), p-β-CAT (p-β-catenin S33/S37/T41), TCF4, c-MYC, WNT1, and CIC-D1 (Cyclin D1) in simple models of EMX1 (E) and EMX2 (F) overexpression with constitutive activation of the Wnt pathway in the AA cell line. G Triple overexpression of EMX1, EMX2 and mutant CTNNB1 in AA cells. H and I Characterization of simple models of overexpression of EMX1 (H) and EMX2 (I) with CTNNB1 in the AW cell line. J, Triple overexpression of EMX1, EMX2 and mutant CTNNB1 in the AW cell line. A Western blot representative experiment of 3 experiments performed independently in triplicate is shown