Fig. 5

Effects of overexpression of EMX1 and EMX2 in combination with constitutive activation of the Wnt pathway. A and B Growth curves for AA cells with overexpression of EMX1 (E1) or EMX2 (E2) and CTNNB1 (β-CAT) (A) or joint overexpression of EMX1 + EMX2 + CTNNB1 (B). C-H Clone formation test performed with the same AA models. In C and F the size of the colonies is indicated. In (D) and (G), the graphs show the number of colonies. In E and H representative images of the clone formation assay plates stained with crystal violet on the last day of the experiment are shown. I-O same for the AW cell line. I, J Growth curves for AW cells with overexpression of EMX1 (E1) or EMX2 (E2) and CTNNB1 (β-CAT) (I) or joint overexpression of EMX1 + EMX2 + CTNNB1 (J). K–O Clone formation test performed with the same AW models. In K and N the size of the colonies is indicated. In L and Ñ the graphs show the number of colonies. In M and O representative images of the clone formation assay plates stained with crystal violet on the last day of the experiment are shown. The mean of a minimum of 3 independent experiments performed in triplicate ± standard deviation are shown. Statistical analysis was performed with Student's t test (* p < 0.05; ** p < 0.01; *** p < 0.001)