Fig. 7
From: Empty spiracles homeobox genes EMX1 and EMX2 regulate WNT pathway activation in sarcomagenesis
Effects of overexpression of EMX1 and/or EMX2 in combination with constitutive activation of the Wnt pathway on stem cell gene expression Quantification of the relative mRNA levels of the OCT4, SOX2, KLF4, MYC, NANOG, NES and PROM1 genes determined by qRT-qPCR in AA cells (A) and AW cells (C) overexpressing EMX1, EMX2 and CTNNB1. Models of joint overexpression of EMX1, EMX2 and CTNNB1 in AA cells (B) and AW cells (D). Graphs of the expression levels (2-ΔCt) for total extracts of each cell line. The mean of a minimum of 3 independent experiments performed in triplicate ± standard deviation are shown. Statistical analysis was performed with Student’s t test (* p < 0.05; ** p < 0.01; *** p < 0.001)