Fig. 2
From: Metabolic impairment of non-small cell lung cancers by mitochondrial HSPD1 targeting
HSPD1 knockdown blocks cell proliferation and clonogenic ability of NSCLC cells. A) Western blot analysis of HSPD1 protein levels in A549, H1299 and H460 cells upon infection with 3 independent shRNAs (#44, #45 and #48) targeting HSPD1 compared to scramble-infected cells (pLKO). β-Actin was used as loading control. B) Real-time proliferation curves of A549, H1299 and H460 infected with non-targeting pLKO or shHSPD1. The cells’ confluency plotted over time is shown. P-values are from two-way ANOVA. Points are averages of biological replicates ± SD. ** < 0.01, **** < 0.0001. Colony formation of A549 (C) and H1299 (D) cells with pLKO or shHSPD1, stained with crystal-violet and quantified in triplicates. Bars are average of biological replicates ± SD. P-values are from unpaired t-test. ** < 0.01, *** < 0.001. FACS plots of A549 (E) and H1299 (F) cells infected with pLKO or shHSPD1 and stained with PI for cell cycle analysis. Bar graph showing the % of cells in each cell cycle phase of A549 (G) and H1299 (H) cells upon infection with pLKO or shHSPD1. P-values are from two-way ANOVA. Bars are average of biological replicates ± SD. * < 0.05, ** < 0.01, *** < 0.001, **** < 0.0001