Fig. 5

Effect of COX-2 and CYP19A1 inhibitor on the mesospheres formation. Spheres MPM were treated with exemestane (EXE) or rofecoxib (ROF) or exemestane and rofecoxib combination (EXE + ROF) Ninety-six hours later, the spheroids were photographed (A) and sphere-forming efficiency (B) and size (C) were determined. All experiments were repeated in triplicate and media values were calculated. Statistically significant effects (paired Student t test P < 0.05) compared to CNTR *, EXE # or ROF §. e IHC staining of COX-2 and E2 in MPP cells after treatment