Fig. 5

Inhibition of MET suppresses tumor PD-L1 expression and reduces tumor growth in immunocompetent mice. A-B Tumor volumes (A) and weights (B) of scrambled negative control (CTRL) and shMET-transfected KPC xenografts in C57BL/6 mice (n = 5). C Staining of CD3 + T cells among CD45 + lymphocyte populations from isolated TILs. D Intracellular cytokine staining of GZMB + and CD8 + cells among CD3 + T cells from purified TILs. Representative images (right) and quantification (left) are shown. E Intracellular cytokine staining of IFNγ + and CD8 + cells among CD3 + T-cells from purified TILs. Representative images (right) and quantification (left) are shown. F Intracellular cytokine staining of TNF-α + and CD8 + cells among CD3 + T cells from purified TILs. Representative images (right) and quantification (left) are shown. TILs, tumor-infiltrating lymphocytes; GZMB, Granzyme B