Fig. 4
RSL1D1 Interacts with HDM2 mRNA to Increase Its Stability. A Downregulation of RSL1D1 increases the decay rate of HDM2 transcripts. siRNA-transfected HCT116p53−/− cells were treated with 4 μM Act-D for 0, 1, 3, and 5 h. The mRNA level of HDM2 was determined by qRT-PCR analysis. GAPDH was used as an internal control to normalize the values. The normalized values of siNC- or siRSL1D1-transfected cells at 0 h were set to 100%. B RIP assay was performed to evaluate the interaction between RSL1D1 and HDM2 mRNA. Anti-FLAG antibody was used for RIP utilizing HCT116p53−/− cells overexpressing FLAG-RSL1D1. Mouse IgG was used as a negative control. The mRNA levels of HDM2 and GAPDH were determined by qRT-PCR. The mRNA levels of input were used to normalize the values. A, B Data are represented as mean ± SD. Student’s t test. *P < 0.05 and **P < 0.01 denote significant difference