Fig. 2

MEN1 silencing triggers the nuclear translocation of JunD in AR-independent PCa cells. Representative images of foci formation assays (a) and their quantification (b) using LNCaP, 22Rv1, DU145 and PC3 cells treated or not with MI503 (2.5 μM). qRT-PCR analysis of JunD and MYC mRNA expression (c) and Western blot analysis showing JunD and MYC expression (d) in 22Rv1 and DU145 cells treated or not with MI503 (2.5 μM). qRT-PCR analysis of JunD mRNA expression (e) and Western blot analysis of JunD protein expression (f) in MEN1-KD 22Rv1 and DU145 cells. g IF staining showing menin and JunD in DU145 cells treated with siCtrl or siMEN1(1) + (3). Scale bar = 25 μm. h Western blot analysis of JunD expression in different subcellular fractions in DU145 and PC3 cells treated with siCtrl or siMEN1(1) + (3). Representative blots of three independent experiments