Fig. 5

JunD and β-catenin are involved in the regulation of EMT marker expression in AR-independent PCa cells. Representative images of soft agar colony formation assay in JunD-KD (a, left panel) and CTNNB1-KD (b, left panel) DU145 or PC3 cells. Scale bar = 50 μm. Graphs showing quantitative analysis of colony formation at 21 days post-transfection with siCtrl or siJunD(1) + (2) using Image J software (right panel). qRT-PCR (c) and Western blot (d) analyses of mRNA and protein expression of HIF-1α, E-cadherin and Twist 1 in JunD-KD DU145 and PC3 cells. qRT-PCR (e) and Western blot (f) analyses of mRNA and protein expression of HIF-1α, E-cadherin and Twist 1 in CTNNB1-KD DU145 and PC3 cells DU145 and PC3 cells. Representative blots of three independent experiments