Fig. 6

The expression of both JunD and β-catenin is critical for the tumorigenic potential of AR-independent PCa cells. a Representative images of foci formation of DU145 cells upon transfection with siMEN1, siJunD, siCTNNB1, siMEN1 + siJunD, siMEN1+ siCTNNB1 or siMEN1 + siJunD+siCTNNB1. Quantification of data is shown in the right and lower panel. b qRT-PCR analysis showing mRNA expression of HIF-1α, E-cadherin, Vimentin, Twist 1 and BMI1 in DU145 and PC3 cells transfected with siCtrl or siMEN1(1) + (3). c Western blot analysis showing total protein expression of menin, HIF-1α, E-cadherin, Vimentin, Twist 1 and BMI1 in DU145 and PC3 cells transfected with siCtrl or siMEN1(1) + (3). d Western blot analysis showing menin, HIF-1α, Vimentin, Twist 1 and BMI1 expression in nuclear and cytoplasmic subcellular fractions in siCtrl or siMEN1(1) + (3)-treated DU145 and PC3 cells. Representative blots of three independent experiments. e qRT-PCR analysis of mRNA expression of E-cadherin, HIF-1α, Twist 1 and Vimentin in PCI-neo or PCI-MEN1-transfected DU145 and PC3 cells. f Western blot analysis of E-cadherin, HIF-1α, Twist 1 and Vimentin protein expression in DU145 and PC3 cells transfected with PCI-neo or PCI-MEN1 as indicated. Representative blots of three independent experiments