Fig. 1

The overexpression of PTK6 is correlated with CRC progression. A-B The expression of PTK6 was analyzed in normal and CRC tissues from TCGA + GTEx and GEO (GDS4382-GPL570) databases. C The expression of PTK6 in stage I-IV CRC samples from GSE39582 dataset. D IHC analysis shows the expression of PTK6 in CRC tissues (T) and adjacent normal mucosa (N) of two representative cases. Bars in the right panel represent the percentage of high and low PTK6 expression patients. E The Kaplan-Meier survival curve shows the correatlation beteween PTK6 expression and overall survival (OS) in CRC patients from the Nanfang cohort. F Western blot analysis shows the expression of PTK6 in fresh CRC tissues (T) and adjacent non-tumor tissues (N). Scatter diagram on the right panel represents relative PTK6 expression in CRC tissues and paired normal tissues. G Western blot, real-time q-PCR and IF assays demonstrate the expression and localization of PTK6 in CRC cells. Scale bar represents 5 μm. *P < 0.05, ***P < 0.001,^# indicates P > 0.05