Fig. 6
XMU-MP-2 inhibits the PTK6 induced proliferation, stemness, and chemoresistance of CRC cells. A The in vitro limiting dilution assays show the effects of XMU-MP-2 on the PTK6 enhanced formation of tumor spheres (mean ± SD, n = 12), likelihood ratio test. B CCK-8 assays were performed to determine the effects of XMU-MP-2 on the PTK6 induced proliferation CRC cells (mean ± SD, n = 3). C Colony formation assays were performed to determine the effects of XMU-MP-2 on PTK6 induced growth of CRC cells. The number of colonies (> 50 cells) was scored (mean ± SD, n = 3). D Dose–response curves show the effects of XMU-MP-2 on the sensitivity of vector, PTK6WT and PTK6YF overexpression CRC cells to chemotherapy drugs (mean ± SD, n = 3). E The numbers of CD133 + cells in indicated CRC cells were evaluated by flow cytometry (mean ± SD, n = 3). F Immunofluorescence staining of CRC cells shows that XMU-MP-2 can inhibit the phosphorylation of PTK6, as well as the activation of its downstream STAT3 signaling. G Immunofluorescence staining of CRC cells and CSC spheres shows that XMU-MP-2 can inhibit the phosphorylation of PTK6 and the expression of stem cell markers. H Western blot assays indicate that XMU-MP-2 can inhibit the PTK6 induced activation of JAK2/STAT3 signaling and the expression of stem cell markers. *P < 0.05, **P < 0.01, ***P < 0.001