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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Phosphorylation of NF-κBp65 drives inflammation-mediated hepatocellular carcinogenesis and is a novel therapeutic target

Fig. 3

Inflammation-induced NF-κBp65 phosphorylation is involved in hepatocellular carcinogenesis. DEN-induced mouse hepatocellular carcinomas were intraperitoneally injected after either physiological saline (vehicle) or a single DEN (15 mg/kg), and the mouse livers were harvested at 9 months after injection. Normal liver tissues were obtained from vehicle mouse livers, HCC tissues from 9-month DEN-induced mouse liver tumours and paracancerous tissues from adjacent noncancerous tissues. a Representative images of p-p65 staining in DEN-induced mouse HCC models. b p65 and p-p65 protein expressions in mouse livers were determined using western blotting. c p65 was analysed in the nuclear and cytoplasmic fractions of normal liver cells isolated from normal control mice and liver tumour cells isolated 9 months after DEN injection. β-actin and histone H3 were used as the controls for loading and fractionation. d, e p65 and p-p65 protein expressions in mouse livers were determined by western blotting after either physiological saline (vehicle) or tetrachloromethane (CCl4) (15 mg/kg) intraperitoneal injection twice per week for 2 months. f Representative images of p65 (top) and p-p65 (bottom) staining in CCl4-induced liver cirrhosis. g, h p65 and p-p65 protein expressions in mouse livers after either physiological saline (vehicle) or 100 mg/kg diethylnitrosamine (DEN) intraperitoneal injection for 10 days were determined using western blotting. i Representative images of p65 (top) and p-p65 (bottom) staining in DEN-induced liver inflammation. j, k p65 and p-p65 protein expressions in mouse livers were determined using western blotting after intraperitoneal TNF-α (40 μg/kg) injection for 6 h. l Representative images of p65 (top) and p-p65 (bottom) staining in TNF-α-induced liver inflammation. Values are mean ± SD (n = 6 in each group). P < 0.05 using Student’s t-test

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