Fig. 10From: FUS-induced circRHOBTB3 facilitates cell proliferation via miR-600/NACC1 mediated autophagy response in pancreatic ductal adenocarcinomaFUS binds to pre-RHOBTB3 mRNA and mediates the biogenesis of circRHOBTB3. a The efficiency of 8 candidate biogenesis-regulating RNA-binding proteins siRNA and relative expression of circRHOBTB3 upon 8 siRNAs transfection compared with negative control. b The anti-FUS RIP assays exhibited that FUS antibody successfully precipitated FUS protein as well as pre-RHOBTB3 mRNA rather than circRHOBTB3, neither RHOBTB3 mRNA in PANC-1 and MiaPaca-2 cell lines. c The pull-down assays were conducted applying pre-RHOBTB3 mRNA probe, circRHOBTB3 probe and negative control probe for further confirmation of the binding relationship between FUS and pre-RHOBTB3. The results revealed that only the pre-RHOBTB3 mRNA probe could successfully pull down FUS protein, verified by western blotting. d The efficiency and specificity of each probe were validated by qRT-PCR e Schematic illustration indicates the mechanism by which circRHOBTB3 promotes autophagy to facilitate malignant progression of PDAC cells via miR-600/NACC1/Akt/mTOR pathway. All data are presented as the means ± SD of three independent experiments. **p < 0.01, ***p < 0.001Back to article page