Fig. 2

circRHOBTB3 promotes the proliferation of PDAC cells in vitro and in vivo. a the efficiency of different siRNAs on cicRHOBTB3 expression level was determined with qRT-PCR in PANC-1 and MiaPaca-2 cell lines. b-c The knocking-down and overexpression efficiency of circRHOBTB3 were detected with qRT-PCR. d The growth curves of PANC-1 and MiaPaca-2 cells were evaluated with CCK-8 assays after knocking-down and overexpression of circRHOBTB3. e Colony formation assays were performed to evaluate cell proliferation. f EdU assays were performed to evaluate cell proliferation abilities. The samples were imaged at 200X magnification. Scale bar = 50 μm. g Representative images of subcutaneous xenograft tumors (n = 6 for each group). Analysis on tumor weight and volume showed decreased tumor growth after knocking-down of circRHOBTB3. h HE and Ki67 staining of xenograft tumors showing inhibited proliferative rate after circRHOBTB3 knockdown. Figures were imaged at 400× magnification. Scale bar = 50 μm. All data are presented as the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001