Fig. 4

Colocalization of GCC185 with S1P and S2P in the prostate tissues. (A and B) IF of S1P (green)/GCC185 (red) (A) and S2P (green)/GCC185 (red) (B) in representative normal prostate tissues, BPH, and PCa tissues with different Gleason scores. All confocal images were acquired with the same imaging parameters; bars, 5 μm. (C and D) Quantification of Pearson coefficient of colocalization in pairs S1P/GCC185 (C) and S2P/GCC185 (D) in the samples described in A and B, respectively (**P < 0.001 between normal, BPH and each group of PCa, as well as between every group of PCa, pairwise Wilcoxon with Bonferonni-Hochberg multiple test). No significant difference was found between normal prostate and BPH. The threshold for GCC185 was normalized for all samples, given the reduction of signal in PCa tissues. (E) Quantification of GCC185 integrated fluorescence intensity (in a.u.) in the representative normal prostate tissues, BPH, and PCa tissues with different Gleason scores presented in A and B. Data are presented as medians (min – max); (**P < 0.001, *P < 0.05, t test)