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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Apatinib triggers autophagic and apoptotic cell death via VEGFR2/STAT3/PD-L1 and ROS/Nrf2/p62 signaling in lung cancer

Fig. 4

Apatinib suppressed the migration and invasion of NSCLC cells. A549 and H1299 cells were treated with apatinib for 48 h. a and b Wound healing assay was performed to evaluate the migration capacity. The migration rate was quantitated. Scar bar = 200 μm. The data are presented as mean ± SD (n = 3). *p < 0.05, **p < 0.01 vs. control. c-e Transwell assays without or with Matrigel were performed to evaluate the migration and invasion capacity. The numbers of migrated and invaded cells were quantitated. Scar bar = 100 μm. The data are presented as mean ± SD (n = 3). *p < 0.05, **p < 0.01 vs. control. f Immunofluorescence staining of E-cadherin and Vimentin (E-cadherin and Vimentin: green; DAPI: blue). Scar bar = 50 μm. g Western blot analysis of EMT-regulated proteins E-cadherin, Vimentin and MMP2

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