Fig. 6

Apatinib induced autophagic and apoptotic cell death via ROS/Nrf2/p62 pathway in NSCLC cells. a The representative images of fluorescence intensity of DCFH-DA in apatinib-treated A549 and H1299 cells. Scale bar = 100 μm. b Western blot analysis of Nrf2 and LC3 expressions in apatinib (10 μM)-treated A549 and H1299 cells for 0–48 h. c Western blot analysis of Nrf2, p62, Beclin1, ATG5, and LC3 expressions in A549 and H1299 cells after various concentrations of apatinib treatment for 48 h. d-f Immunofluorescence staining of Nrf2 (d), p62 (e), and LC3 (f) in A549 and H1299 cells after indicated treatment (Nrf2, p62, and LC3: green; DAPI: blue). Scar bar = 50 μm. g Western blot analysis of Nrf2, p62, LC3, Cyclin D1 and Cleaved Caspase 3 expressions in A549 and H1299 cells after apatinib with or without NAC treatment for 48 h