Fig. 3

SIRT1 knockdown impairs proliferation. a SIRT1 protein levels were analyzed in MOLT-4 and CCRF-CEM cells infected with ShRNA. b ShSIRT1 and ShSCR cells were counted and plotted at the indicated time points(n = 3). c The left panel showed representative of colonies of ShSIRT1 and ShSCR cells (scale = 200 μm). The right panel showed colony numbers of three independent trials. d Cell cycle phase distribution of ShSIRT1 cells were analyzed by FACS and quantified (n = 3). e Apoptotic cell death was analyzed by Annexin V-PI staining and quantified (n = 3). f Western blotting for SIRT1 expression in cells transduced with shSIRT1–1 and empty vector (Vector), coding sequences of SIRT1 (CDS) or SIRT1 deacetylase mutant (SIRT1-HY). g Cell growth were counted and plotted at the indicated time points(n = 3)