Fig. 4

SIRT1 knockout significantly decreases leukemia burden in the murine T-ALL model. a Schematic representation of murine T-ALL model. b Peripheral blood (PB) percentage of GFP⁺ cells (n = 10 per group) at the indicated time points. c Cell counts of WBC in the PB of recipient mice at 4 weeks after transplantation. d-e Quantification of GFP⁺ cell infiltration in the bone marrow and spleen of mice 4 weeks after transplantation (n = 5 per group) f Kaplan-Meier survival curves of mice harboring SIRT1 KO and WT T-ALL (n = 10 per group) g-i Representative images and quantification of the spleens and livers. j Histological analysis of livers, spleens and bones (scale = 5 mm). k Kaplan-Meier survival curves of mice upon the second transplantation (cell dose = 1 × 10⁶ GFP⁺ cells per mouse, n = 10 per group). l-m Fraction of secondary recipients that developed leukemia when transplanted with limiting dilutions of GFP⁺ cells. n Representative images of the cell cycle status in WT and KO T-ALL. o Results of cell cycle phase distribution in WT and KO T-ALL (n = 5 per group)