Fig. 6

Inhibition of SIRT1 reduces T-ALL cell growth by activating p27. a SIRT1 and p27 protein levels were analyzed in MOLT-4 or CCRF-CEM cells co-transduced with ShSIRT1 and Shp27. b MOLT-4 (top panel) and CCRF-CEM (bottom panel) co-transduced with ShSIRT1 and Shp27 were counted at the indicated time points(n = 3). c-d Cell cycle phase distribution of MOLT-4 or CCRF-CEM cells co-transduced with ShSIRT1 and Shp27 were analyzed by FACS and quantified (n = 3). e Schematic representation of murine T-ALL model co-expressing Shp27. f Survival was compared among the recipient mice in e. g-h Representative images and weight quantification of spleens and livers. i-j Cell cycle phase distribution of WT and KO T-ALL expressing Shp27 were analyzed by FACS and quantified (n = 5 per group)