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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: SIRT1 regulates the phosphorylation and degradation of P27 by deacetylating CDK2 to promote T-cell acute lymphoblastic leukemia progression

Fig. 7

SIRT1 promotes ubiquitination and Thr-187 phosphorylation of p27. a 293 T cells were co-transfected with pCMV-N-Flag (FV), Flag-p27, pCMV-SKP2, pCMV-N-MycTag (MV), MycTag-SIRT1 (S), MycTag-SIRT1-H363Y (HY) and HA-tagged ubiquitin as indicated and subjected to ubiquitination analysis. b-e Western blot relative quantification analysis of Phospho-p27 and p27 levels in ShSIRT1 MOLT-4 and CCRF-CEM cells. f-g Western blotting for Flag-p27 or Flag-p27T187A following CHX treatment in cells from Fig. S7c or d (left panel). The right panel showed results of densitometry quantitation (n = 3)

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