Fig. 6

NMU secretion, colony formation and morphology of NMU-overexpressing HT29 and Caco-2 clones. A NMU secretion by HT29 (left) and Caco-2 (right) NMU-overexpressing clones was confirmed by NMU immunoprecipitation from the cell-conditioned media. The bands were quantified by densitometry. The results are shown as the median with min-to-max range (*p ≤ 0.05, ***p ≤ 0.001; n = 3). The images (upper panel) show representative results. B Clonogenic capabilities of the NMU-overexpressing clones. The images (upper panel) show representative colony densities. The results are shown as the median with interquartile range (*p ≤ 0.05, **p ≤ 0.01; n ≥ 3). C Morphology of the HT29 and Caco-2 NMU-overexpressing clone colonies. HT29 pcDNA and Caco-2 pcDNA – control clones transfected with an empty vector; HT29 NMU1, HT29 NMU45, Caco-2 NMU1, Caco-2 NMU3 – various clones overexpressing NMU