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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Neuromedin U induces an invasive phenotype in CRC cells expressing the NMUR2 receptor

Fig. 7

Migration and invasiveness of colorectal cancer cells overexpressing NMU. A HT29 NMU and B Caco-2 NMU clone migration (left panel) and invasion (right panel) capabilities compared with those of control pcDNA clones. The results are shown as the mean with SD (*p ≤ 0.05, **p ≤ 0.01; n ≥ 3). The images show representative results. ERK1/2 kinase activation in the Caco-2 NMU clones treated with the ERK1/2 phosphorylation inhibitor PD89059 was analysed by immunoblotting. The image shows a representative result. The bands were quantified by densitometry. The intensity of the pERK1/2 bands was normalized against that of the respective ERK1/2 and α-tubulin bands. The results are shown as the median with min-to-max range (*p ≤ 0.05, **p ≤ 0.01; n ≥ 4). D Caco-2 NMU1 clone migration was inhibited by the PD89059 inhibitor. The results are shown as the mean with SD (*p ≤ 0.05; n = 4). The images show representative results. HT29 pcDNA and Caco-2 pcDNA – control clones transfected with an empty vector; HT29 NMU1 and HT29 NMU45, Caco-2 NMU1 and Caco-2 NMU3 – various clones overexpressing NMU

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