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Fig. 4 | Journal of Experimental & Clinical Cancer Research

Fig. 4

From: Circ-GALNT16 restrains colorectal cancer progression by enhancing the SUMOylation of hnRNPK

Fig. 4

Circ-GALNT16 is mainly localized in the nucleus and binds to the KH3 domain of hnRNPK. a, b. Subcellular fractionation and FISH assays indicated that circ-GALNT16 was predominately localized in the nucleus of CRC cells. c-e. RNA pulldown assay followed by silver staining, MS, and RIP assay indicated that circ-GALNT16 specifically interacted with hnRNPK. f. Dual RNA FISH and immunofluorescence assays showed that circ-GALNT16 and hnRNPK colocalized in the nucleus of DLD-1 and LoVo. g. Functional domain and truncated mutation annotation of hnRNPK. h-i. RIP assay and RNA pulldown assay confirmed that circ-GALNT16 characteristically interacted with the KH3 domain of hnRNPK. j. The protein level of hnRNPK in circ-GALNT16 knockdown and overexpression cells. k. The circ-GALNT16 expression level in hnRNPK silencing cells.nsp > 0.05, **p < 0.01

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