Fig. 3

Acquisition of stemness is mediated by Spy1-CLIP3 axis in GBM cells. A mRNA levels of Spy1, NANOG, and OCT4 were analyzed by real-time qRT-PCR upon Spy1 siRNA treatment in U87MG, T98G, and GSC11 cells. B mRNA levels of CLIP3, NANOG, and OCT4 were analyzed by real-time qRT-PCR upon CLIP3 siRNA treatment in U87MG, T98G, and GSC11 cells. C In vitro limiting dilution assays of U87MG, T98G, and GSC11 cells treated with Spy1 or CLIP3 siRNA. The frequency of GSCs was calculated by extreme limiting dilution assay (ELDA) analysis. *p < 0.05, **p < 0.01, ***p < 0.001 with unpaired t-test