Fig. 5

Spy1-CLIP3 axis contributes to metabolic shift toward glycolysis in GBM cells. A, B OCR and ECAR were measured by Seahorse analyzer in U87MG, T98G, and GSC11 cells. Basal OCR indicates the basal level of oxygen consumption and ATP production indicates the decrease in oxygen consumption rate upon injection of oligomycin, which represents the portion of basal respiration that was being used to drive ATP production. Glycolysis indicates the generation of lactate upon glucose addition and glycolytic capacity indicates the maximum capacity of lactate generation upon inhibition of oxidative phosphorylation. OCR and ECAR upon transfection of Spy1 siRNA (A) or CLIP3 siRNA (B). *p < 0.05, **p < 0.01, ***p < 0.001 with unpaired t-test