Fig. 2

Y10 phosphorylation of LDHA is required for cyclin G2-mediated antitumor functions. a Western blotting was performed to examine LDHA expression and Y10 phosphorylation of LDHA in LDHA-knockdown U87 cells with either exogenous LDHA WT or LDHA Y10F expression following cyclin G2 overexpression. b-c Lactate production and glucose uptake measured in LDHA-knockdown U87 cells with either exogenous LDHA WT or LDHA Y10F expression following cyclin G2 overexpression. d The effect of cyclin G2 overexpression on proliferation of LDHA-knockdown U87 cells with either exogenous LDHA WT or LDHA Y10F expression by MTS. e-f The effect of cyclin G2 overexpression on colony formation of LDHA-knockdown U87 cells with either exogenous LDHA WT or LDHA Y10F expression by colony formation assay. g-i The effect of cyclin G2 overexpression on migration and invasion capacity of LDHA-knockdown U87 cells with either exogenous LDHA WT or LDHA Y10F expression by cell migration and invasion assay. j-k Flow cytometry results showing the influence of cyclin G2 overexpression on apoptosis in LDHA-knockdown U87 cells with either exogenous LDHA WT or LDHA Y10F expression. Data of triplicate samples from three independent experiments are presented as the mean ± s.d. ^*p < 0.05; ^**p < 0.01; ^***p < 0.001; ns, not significant