Fig. 3From: Cyclin G2 reverses immunosuppressive tumor microenvironment and potentiates PD-1 blockade in gliomaCyclin G2 knockout resulted in an immunosuppressive microenvironment. a Schematic depicting experimental setup. WT and Ccng2−/− mice were subcutaneously (s.c.) injected with GL261 cells and treated with AZD3965 or vehicle by oral gavage (p.o.). Gross tumors (b), tumor growth curves (c), tumor volume (d) and tumor weight (e) at endpoint were measured. n = 5. Scale bar, 1 cm. f The concentration of lactate in tumors from each group. g-l The frequency of Treg, IFN-γ + CD4 + T, IFN-γ + CD8 + T cells in tumors of each group as measured with flow cytometry. n = 4 for each group. m-p Levels of IFN-γ, TNF-α, TGF-β and IL-10 in tumors of each group determined by ELISA. Data are presented as the mean ± s.d. *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significantBack to article page