Fig. 4

Cyclin G2 mediated immunomodulation via NF-κB signaling. a-b Naïve CD4 + T cells were stimulated and cultured in RPMI-1640 and conditioned medium from GL261-NC or GL261-KD for Treg polarization. The frequency of Treg was determined by flow cytometry. c-d Levels of TGF-β and IL-10 in the supernatant were determined by ELISA. e Expression levels of proteins involved in NF-κB signaling in CD4 + T cells as quantified by western blotting. f Results of the cytotoxicity of CTLs as determined by CytoTox96® Non-Radioactive Cytotoxicity Assay. g-h Levels of IFN-γ and TNF-α in the supernatant as determined by ELISA. Data of triplicate samples from four independent experiments are presented as the mean ± s.d. ^*p < 0.05; ^**p < 0.01; ^***p < 0.001; ns, not significant