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Fig. 2 | Journal of Experimental & Clinical Cancer Research

Fig. 2

From: Long non-coding RNA SLC2A1-AS1 induced by GLI3 promotes aerobic glycolysis and progression in esophageal squamous cell carcinoma by sponging miR-378a-3p to enhance Glut1 expression

Fig. 2

Transcriptional factor GLI3 binds to the promoter region of SLC2A1-AS1 and manipulates its expression in ESCC cells. A. Three predicted GLI3 binding sites on SLC2A1-AS1 promoter by hTFtarget and JASPAR online software. B and C. The luciferase reporter assay of pGL3-basic SLC2A1-AS1 promoter vector with WT or MUT GLI3 binding sites in EC9706, TE1 and KYSE180 cells transfected with pcDNA3.1 or pcDNA3.1-GLI3 vector. D. StarBase online software assay for GLI3 expression in ESCA tissues. E. TCGA assay for GLI3 expression level in ESCC samples and normal samples. F. GEO dataset GSE111011 assay for GLI3 expression in 7 cases of ESCC tissues and paired normal tissues. G. StarBase online software assay for the correlation between GLI3 expression and SLC2A1-AS1 expression in ESCA samples. H. The detection of GLI3 expression after transfection with GLI3 siRNAs in ESCC cells. I. GLI3 expression assay after transfection with pcDNA3.1-GLI3 vector in ESCC cells. J. GLI3 siRNA markedly downregulates the SLC2A1-AS1 expression in ESCC cells. K. GLI3 overexpression significantly upregulates the SLC2A1-AS1 level in ESCC cells. Compared to pcDNA3.1 or con-siRNA group, **P < 0.01, ***P < 0.001 and ****P < 0.0001, indicating statistical significance

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