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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Long non-coding RNA SLC2A1-AS1 induced by GLI3 promotes aerobic glycolysis and progression in esophageal squamous cell carcinoma by sponging miR-378a-3p to enhance Glut1 expression

Fig. 3

SLC2A1-AS1 depletion contributes to the suppression of cell proliferation and the induction of cell apoptosis in ESCC cells. A. qRT-PCR was used to determine SLC2A1-AS1 expression following transfection with SLC2A1-AS1 siRNA in ESCC cells. B. CCK-8 assay for cell proliferation at 24 h, 48 h, 72 h and 96 h after transfection with SLC2A1-AS1 siRNA in ESCC cells. C. SLC2A1-AS1 downregulation markedly reduced colony formation in ESCC cells. D. Statistical assay for the number of colony formation in ESCC cells treated with NC or SLC2A1-AS1 siRNA. E. EdU staining assay for EdU-positive cell numbers in ESCC cells treated with NC or SLC2A1-AS1 siRNA. F. Quantitative assay of the ratio of EdU-positive cell numbers in ESCC cells treated with NC or SLC2A1-AS1 siRNA. G. Flow cytometry detection for cell apoptosis in different treatment ESCC cells. H. Quantitative analysis for apoptotic cell numbers in a variety of ESCC cells. Compared to NC group, *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001, indicating statistical significance

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