Fig. 3

SLC2A1-AS1 depletion contributes to the suppression of cell proliferation and the induction of cell apoptosis in ESCC cells. A. qRT-PCR was used to determine SLC2A1-AS1 expression following transfection with SLC2A1-AS1 siRNA in ESCC cells. B. CCK-8 assay for cell proliferation at 24 h, 48 h, 72 h and 96 h after transfection with SLC2A1-AS1 siRNA in ESCC cells. C. SLC2A1-AS1 downregulation markedly reduced colony formation in ESCC cells. D. Statistical assay for the number of colony formation in ESCC cells treated with NC or SLC2A1-AS1 siRNA. E. EdU staining assay for EdU-positive cell numbers in ESCC cells treated with NC or SLC2A1-AS1 siRNA. F. Quantitative assay of the ratio of EdU-positive cell numbers in ESCC cells treated with NC or SLC2A1-AS1 siRNA. G. Flow cytometry detection for cell apoptosis in different treatment ESCC cells. H. Quantitative analysis for apoptotic cell numbers in a variety of ESCC cells. Compared to NC group, *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001, indicating statistical significance