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Fig. 5 | Journal of Experimental & Clinical Cancer Research

Fig. 5

From: Long non-coding RNA SLC2A1-AS1 induced by GLI3 promotes aerobic glycolysis and progression in esophageal squamous cell carcinoma by sponging miR-378a-3p to enhance Glut1 expression

Fig. 5

SLC2A1-AS1 competitively absorbs miR-378a-3p by ceRNA mechanism in ESCC cells. A. qRT-PR assay of subcellular SLC2A1-AS1 level in the nucleus and cytoplasm of EC9706, TE1 and KYSE180 cells, GAPDH and U6 are employed as endogenous controls. B. Subcellular localization of SLC2A1-AS1 in EC9706, TE1 and KYSE180 cells is investigated by FISH experiment, SLC2A1-AS1 is labeled by Cy3 (red) and nuclei are stained with DAPI (blue). C. The miR-378a-3p binding sites in SLC2A1-AS1 transcript are predicted by DIANA LncBase Predicted v.2. D. The correlation of SLC2A1-AS1 with miR-378a-3p is examined in ESCA samples using the bioinformatics tool StarBase E. Double luciferase report experiment is performed to verify the interaction of SLC2A1-AS1 with miR-378a-3p by co-transfecting pmirGLO-SLC2A1-AS1-WT or pmirGLO-SLC2A1-AS1-MUT along with NC-mimic or miR-378a-3p mimic into ESCC cells, compared to NC group, **P < 0.01 and ****P < 0.0001, indicating statistical significance. F. Fold enrichment of SLC2A1-AS1 by Ago2 antibody or IgG in EC9706, TE1 and KYSE180 cells. G. Fold enrichment of miR-378a-3p by Ago2 antibody or IgG in EC9706, TE1 and KYSE180 cells. H. Enrichment of SLC2A1-AS1 in EC9706, TE1 and KYSE180 cells transfected with NC mimic or miR-378a-3p mimic, (F, G and H, compared to IgG group, ****P < 0.0001, indicating statistical significance). I. SLC2A1-AS1 depletion promotes the expression of miR-378a-3p in EC9706, TE1 and KYSE180 cells compared to NC group, ****P < 0.0001, indicating statistical significance. J. SLC2A1-AS1 overexpression suppresses the expression of miR-378a-3p in EC9706, TE1 and KYSE180 cells, compared to pcDNA3.1, ****< 0.0001, indicating statistical significance

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