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Fig. 6 | Journal of Experimental & Clinical Cancer Research

Fig. 6

From: Long non-coding RNA SLC2A1-AS1 induced by GLI3 promotes aerobic glycolysis and progression in esophageal squamous cell carcinoma by sponging miR-378a-3p to enhance Glut1 expression

Fig. 6

The expressions of miR-378a-3p and Glut1, and Glut1 is a direct target of miR-378a-3p in ESCC cells. A. The expression of miR-378a-3p is detected in 162 cases of ESCA samples and 11 cases of normal samples by StarBase online software. B. The miR-378a-3p level is investigated in 119 cases of ESCC tissues and paired normal tissues by GEO dataset GSE43732. C. The miR-378a-3p level is determined in 60 cases of ESCC tissues and paired normal tissues by qRT-PCR, compared to normal tissues, ****P < 0.0001, indicating statistical significance. D. The expression of miR-378a-3p is examined by qRT-PCR in a panel of ESCC cells, compared to Het-1A cell, **P < 0.01, ***P < 0.001 and ****P < 0.0001, indicating statistical significance. E. The expression of Glut1 is detected in 162 cases of ESCA samples and 11 cases of normal samples by StarBase online software. F. The Glut1 level is investigated in 7 cases of ESCC tissues and paired normal tissues by GEO Dataset GSE111011. G. Western blot assay for Glut1 protein expression in a panel of ESCC cells and normal esophageal epithelial cell Het-1A. H. Quantification of relative levels of Glut1 in a number of ESCC cells and normal esophageal epithelial cell Het-1A, compared to Het-1A cell, *P < 0.05, **P < 0.01 and ****P < 0.0001, indicating statistical significance. I. IHC assay for Glut1 protein expression in 60 cases of ESCC tissues and paired normal tissues. J. The miR-378a-3p binding sites in Glut1 transcript are predicted by miRDB online software. K. Double luciferase report experiment is carried out to confirm the interaction of Glut1 with miR-378a-3p by co-transfecting pmirGLO-Glut1-WT or pmirGLO-Glut1-MUT as well as NC-mimic or miR-378a-3p mimic into ESCC cells. L. miR-378a-3p mimic upregulates the expression of miR-378a-3p, whereas miR-378a-3p inhibitor downregulates the miR-378a-3p level in ESCC cells. M. miR-378a-3p mimic suppresses the expression of Glut1 protein in ESCC cells. N. The analysis of relative level of Glut1 protein after transfection with NC mimic or miR-378a-3p mimic in ESCC cells. O. miR-378a-3p inhibitor enhances the expression of Glut1 protein in ESCC cells. P. The assay of relative level of Glut1 protein after transfection with NC inhibitor or miR-378a-3p inhibitor in ESCC cells. (K, L and N, compared to NC-mimic or NC-inhibitor, **P < 0.01, ***P < 0.001 and ****P < 0.0001, indicating statistical significance)

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