Fig. 7

miR-378a-3p mimic mediated biological effects is dependent on the SLC2A1-AS1 and Glut1 expression in ESCC cells. A. ESCC cell proliferation was assessed by CCK-8 assay in NC mimic group, miR-378a-3p mimic group, miR-378a-3p plus pcDNA3.1-SLC2A1-AS1 group and miR-378a-3p plus pcDNA3.1-Glut1 group. B. Cell apoptosis was determined by Flow cytometry in the same groups appeared in cell proliferation. C. Quantitative assay of apoptotic cell numbers in different treatment ESCC cells. D. Cell invasion ability was detected using Transwell chamber in various ESCC cells with different groups. E. Quantification of invasive cell numbers in diverse ESCC cells with various groups. F and G. The determination of glucose consumption (F) and lactate production (G) in different groups. H. Western blot assay for HK2, PFKM, PKM and LDHA protein expressions in diverse groups. I. Quantification of the relative levels of HK2, PFKM, PKM and LDHA proteins in different treatment groups. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 were regarded as statistical significance. J. A proposed scheme of the roles and molecular mechanisms of SLC2A1-AS1 in ESCC progression and glycolysis is presented. Note: 1: NC mimic; 2: miR-378a-3p mimic; 3: miR-378a-3p plus SLC2A1-AS1; 4: miR-378a-3p plus Glut1