Fig. 7

SHOX2 drives breast cancer metastasis in a WASF3-dependent manner. (a-d) The effect of WASF3 overexpression on tumor growth repressed by SHOX2 knockdown in MDA-MB-231-bearing NSG mice (n = 5/group). Tumor weight, growth curves, representative Ki67 immunostaining, and quantitative data of Ki67-positive cells are shown in (a), (b), (c), and (d), respectively. (e, f) The effect of WASF3 overexpression on lung metastasis repressed by SHOX2 knockdown in MDA-MB-231-bearing NSG mice. The number of nodules on the lung surface and metastasis index measured on H&E-stained lung sections are shown in (e) and (f), respectively. (g) Proposed model for the transcriptional regulation of WASF3 by SHOX2/STAT3 signaling in breast cancer cells. ^*p < 0.05, ^**p < 0.01