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Fig. 3 | Journal of Experimental & Clinical Cancer Research

Fig. 3

From: Radiation induces NORAD expression to promote ESCC radiotherapy resistance via EEPD1/ATR/Chk1 signalling and by inhibiting pri-miR-199a1 processing and the exosomal transfer of miR-199a-5p

Fig. 3

a OD450 values of several H3 histone proteins in KYSE-150R and KYSE-150 cells. One hundred nanograms of total histone proteins per well were used. b The level of H3K4me2 measured in KYSE-150 and TE-1 cells at 0, 12, 24, and 48 h post 8 Gy X-ray radiation treatment. c The level of H3K4me2 measured in KYSE-150 and TE-1 cells at 24 h after 8 Gy X-ray radiation or 0 Gy X-ray treatment. d The expression level of NORAD in patients with ESCC presenting KMT2A, KMT2B, KMT2C and KMT2D mutant type (MT) or wild type (WT) based on TCGA datasets. e The expression level of H3K4me2 measured in KYSE-150 and TE-1 cells with or without ASH2L and RbBP5 knockdown. f qRT-PCR measurement of the NROAD expression in KYSE-150 and TE-1 cells with or without ASH2L knockdown, which were treated with 8 Gy X-rays treatment. g ChIP-qPCR analysis of NORAD and H3K4me2 were performed in KYSE-150 and KYSE-150R cells and immunoprecipitated using H3K4me2; IgG was taken as a negative control. (qRT-PCR results quantification relative to GAPDH, n = 3 technical replicates, *P < 0.05, **P < 0.01, *** P < 0.001, ****P < 0.0001)

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