Fig. 4

a Replication fork recovery was assayed as the percentage of cells with positive BrdU foci at 30 min after 8 Gy X-ray treatment. Representative immunofluorescence images are shown for TE-1 and KYSE-150 cells transfected with SH-NORAD or SH-NC. Image magnification (400×). b The percentage of BrdU-positive cells was quantified and analysed. c The cell cycle distribution of KYSE-150 cells transfected with SH-NORAD or SH-NC was measured using flow cytometry. Cells were exposed to 8 Gy of X-ray radiation or mock treatment. d The percentage of cells in each cell cycle phase in Fig. 4(c) is shown. e KYSE-150 and TE-1 cells were transduced with an I-SceI expression plasmid to induce DSBs, and homologous recombination repair in NORAD knockdown cells and control cells was assayed using flow cytometry and presented as the percentage of GFP-positive cells. f The GFP-positive percentage of each cell was quantified and analysed. g Cell apoptosis rates in KYSE-150 and TE-1 cells transfected with SH-NC or SH-NORAD and exposed to 8 Gy radiation or mock treatment were measured using flow cytometry. h The cell apoptosis rates were analysed. (n = 3 technical replicates, *P < 0.05, **P < 0.01, *** P < 0.001, ****P < 0.0001)