Fig. 6

a Heatmap of the differentially expressed microRNAs between SH-NC exosomes and SH-NORAD exosomes. b The numbers of up- or downregulated microRNAs in SH-NORAD exosomes compared with SH-NC exosomes. c Scatter plot showing the expression of microRNAs in SH-NC exosomes and SH-NORAD exosomes. d qRT–PCR evaluation of the top 5 differentially expressed microRNAs (miR-615-3p, miR-1306-5p, miR-199a-5p, miR-628-3p, and miR-222-5p) in SH-NC-KYSE-150 cells and SH-NORAD-KYSE-150 cells. e qRT–PCR evaluation of the expression of miR-199a-5p in SH-NC exosomes and SH-NORAD exosomes. f qRT–PCR evaluation of the expression of miR-199a-5p in radioresistant ESCC cells (KYSE-150R and TE-1R) and their parental cells (KYSE-150 and TE-1). g qRT–PCR evaluation of the expression of miR-199a-5p in radioresistant ESCC cells (KYSE-150R and TE-1R) that were cocultured with or without SH-NORAD exosomes. h KYSE-150 cells were transduced with miR-199a-5p mimics or miR-199a-5p nc. Cells were exposed to 0, 2, 4, 6, 8 Gy of X-rays. Colonies were stained and images were captured after 14 days. i The survival fraction of KYSE-150R which were transduced with miR-199a-5p mimics or miR-199a-5p nc were calculated and analysed. (n = 3 technical replicates, *P < 0.05, **P < 0.01, *** P < 0.001, ****P < 0.0001. qRT–PCR results for miRNAs were quantified relative to U6)