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Fig. 7 | Journal of Experimental & Clinical Cancer Research

Fig. 7

From: Chemoresistance is mediated by ovarian cancer leader cells in vitro

Fig. 7

Leader cells express markers indicative of a “stemness” profile. Cells were sorted by flow cytometry based upon LC status, mRNA was extracted and cDNA synthesized. Gene expression of A KRT14, B CD44v6, C ALDHI, D Twist, E WNT and F Nanog was analyzed by quantitative real-time PCR. Data was normalised to ribosomal 18S and graphed as the mean value ± SEM with a single representative real time experiment shown. Analysis was performed by one-way ANOVA and Tukey’s multiple comparison post hoc test to determine statistically significant differences between groups (* p < 0.05, ** p < 0.01) (n = 3 separate isolations in a single run)

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