Fig. 6

ALKBH5-HOXA10 loop overexpression activates the JAK2/STAT3 signaling pathway. (a) Upregulation of the ALKBH5-HOXA10 loop promotes JAK2 expression and the phosphorylation level of STAT3. (b) Downregulation of the ALKBH5-HOXA10 loop inhibits JAK2 expression and the phosphorylation level of STAT3. (c) Activation of the JAK2/STAT3 pathway can be rescued by knocking down ALKBH5 or HOXA10 respectively in cells with HOXA10 and ALKBH5 overexpression. (d and e) The IHC assays show ALKBH/HOXA10/JAK2 regulation in the xenograft model