Fig. 5
In vivo distribution of cryopreserved pNK cells. a-b. Cryopreserved pNK cells (1 × 107 cells) were intravenously injected into Balb/c nude mice. Mice were sacrificed at 2, 24, 48, 72, and 168 h (n = 3 per time point) (a). The numbers of intravenously injected NK cells calculated from the amount of human genomic DNA extracted from the blood, lungs, spleen, kidney, liver, bone marrow, heart, and ovary were quantified using Alu qPCR (b). Data are represented as mean ± SD. c-e. Expanded pNK cells (1 × 107 cells) were intravenously injected into A2780 tumor-bearing NSG mice after 40 days of tumor inoculation. Tumor were resected 2, 24, and 72 h after injection of pNK cells (n = 3 per time point) (c). The number of intravenously injected pNK cells was calculated from the amount of human genomic DNA extracted from the tumors using Alu qPCR (d). Immunofluorescence staining for transferred cells (CFSE, green) and nuclei (DAPI, blue) in tumor tissues (e). Scale bars, 100 μm. Left panel: Representative images of intravenously pNK injected mice tumor. Right panel: The number of pNK cells in tumors was measured (n = 3 per group). Data are represented as mean ± SD