Fig. 6
In vivo therapeutic effects of cryopreserved pNK cells against cisplatin-resistant A2780cis xenograft tumors. a. Experimental design for cisplatin and pNK cell treatment of xenograft mouse models bearing A2780cis cells. Cisplatin (1 mg/kg) was intraperitoneally injected into mice once a week (red arrow), and 1 × 107 pNK cells were intravenously injected into mice on the indicated day (blue arrow) (n = 8–9 per each group). b. Tumor volume of A2780cis was measured at the indicated days after tumor injection. Statistical analysis was performed using two-way ANOVA (**P < 0.01 and ****P < 0.0001 as compared to the control group). The results are expressed as average ±SEM. c. Tumor weight was measured after the mouse was sacrificed. Left panel: Representative image of A2780cis tumor from each group mice sacrificed after 40 days of tumor injection. Right panel: Average tumor weight with SD (n = 8 per group). Each group was compared to the control. Statistical analysis was performed using an unpaired t-test (**P < 0.01). d. Interferon-γ levels in plasma samples from each group of mice. Plasma was harvested at Day 40 post-tumor injection. Each group was compared to the control. Statistical analysis was performed using an unpaired t-test (*P < 0.05)